How Much Sybr Green To Add To Gel. Shipped on gel packs storage conditions: 2.1 prepare the agarose gel directly in sybr ™ safe dna gel stain.
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For best results, add 1 µl of of the 10,000× dsgreen solution in dmso per each 10 ml of buffer near the anode («+», red wire). Yes, you can stain your tbe gels with ethidium bromide, sybr green i, sybr green ii, and the silverxpress silver staining kit. Sybr safe stain is provided in buffer;
Sybr Safe Stain Specifically Formulated To Be A Safer Alternative To Ethidium Bromide.
Both methods are appropriate but for better results, i suggest add sybr green at the time of casting gel. It is an asymmetric cyanine dye that largely binds to the minor groove of dsdna, independent of the nucleotide sequence. It has a peak fluorescence.
For Ethidium Bromide Staining, Soak The Gel In A 2 Îœg/Ml Solution Of Ethidium Bromide In Ultrapure Water For 20 Minutes.
Sybr ® green can also be used to visualize dna in electrophoresis gels. Allow the gel to cool to around 50 °c before adding the dye. Sybr ® green i is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids.
Shipped On Gel Packs Storage Conditions:
For in vitro use only!. With 300 nm transillumination, as little as 60 pg dsdna per band can be detected with sybr® green i stain. What better with a 1:5000 dilution of sybr green ii stain.
If Using The 10,000X Sybr™ Safe Stain Concentrate, Dilute The Buffer Plus Stain Mixture To The Powdered Agarose.
Sybr ® green has a high specificity for dsdna and is especially useful when rna or ssdna may also. Remove the agarose gel and casting tray from the electrophoresis chamber. Laminar flow hood for pcr set up (optional) supplies.
View Or Document The Gel Using Available Light Source And An Green/Yellow Filter.
Plus, when used with blue light, safe dna. 10,000 by adding 7.5 μl of the concentrated stain to 75 ml of 1x electrophoresis buffer in a flask. Slide the gel off of the casting.
